Summary of Reverse Genetics Successes

Locus Heritable Alleles Presumed Function Reference
Crs1 3 Required for splicing of chloroplast atpF intron RNA. 2001 Sep;7(9):1227-38.
CRS1 is a novel group II intron splicing factor that was derived from a domain of ancient origin. Till B, Schmitz-Linneweber C, Williams-Carrier R, Barkan A.
Caf1 2 Member of CRM domain family, involved in chloroplast group IIB intron splicing EMBO J. 2003 Aug 1;22(15):3919-29.
Group II intron splicing factors derived by diversification of an ancient RNA-binding domain.Ostheimer GJ, Williams-Carrier R, Belcher S, Osborne E, Gierke J, Barkan A.
Caf2 1 Member of CRM domain family, involved in chloroplast group IIB intron splicing EMBO J. 2003 Aug 1;22(15):3919-29.
Group II intron splicing factors derived by diversification of an ancient RNA-binding domain.Ostheimer GJ, Williams-Carrier R, Belcher S, Osborne E, Gierke J, Barkan A.
PPR2 2 PPR protein: chloroplast gene-expression Plant J. 2003 Dec;36(5):675-86.
A chloroplast-localized PPR protein required for plastid ribosome accumulation.
Williams PM, Barkan A.
PPR4 2 PPR protein: chloroplast gene-expression  
PPR5 2 PPR protein: chloroplast gene-expression  
RNC 4 RNase III domain, chloroplast gene-expression.  
Csr1 3 A chloroplast signal recognition particle (SRP) that is related to the SRP involved in secretion in bacteria. Plant Cell. 2004 Jan;16(1):201-14. Epub 2003 Dec 19.
Maize mutants lacking chloroplast FtsY exhibit pleiotropic defects in the biogenesis of chloroplast membranes. Asakura Y, Hirohashi T, Kikuchi S, Belcher S, Osborne E, Yano S, Terashima I, Barkan A, Nakai M.
Tha8 2 Involved in delta-pH mediated chloroplast targeting pathway. manuscript in preparation
ChlH 2 Chlorophyll synthesis  
cpx1 1 a gene involved in chlorophyll/heme biosynthesis  
p24 2 plastid - localized member of "whirly transcription factor" family  
VDL helicase 2 chloroplast Dead box protein  
PPR100 2 chloroplast PPR protein  
PPR103 2 chloroplast PPR protein  
PPR7 2 chloroplast PPR protein  
MRT 2 coimmunoprecipitates with chloroplast group II intron complexes  
Last modified on July 12, 2006