These are some of our better characterized mutants...

Seed may be requested from either request@pml.uoregon.edu , or the maize co-op.

Locus Mapping Data Allele Alternate allele name Pigment Phenotype Chloroplast Protein Phenotype Accession Publication Comments
atp1 atp1-1 slightly pale green specifically lacks the plastid ATP synthase McCormac and Barkan, 1999, Plant Cell 11: 1709-1716
caf1 caf1-1 ivory null allele AY264368 Ostheimer et al, 2003, EMBO 22:3919-29 Mu- induced allele; CRS2-associated factor; mutant has defect in chloroplast splicing. Barkan lab, unpublished
caf1 caf1-2 ivory/ very pale yg leaky allele AY264368 Ostheimer et al, 2003, EMBO 22:3919-29 Mu- induced allele; CRS2-associated factor; mutant has defect in chloroplast splicing. Barkan lab, unpublished
caf2 caf2-1 ivory AY264369 Ostheimer et al, 2003, EMBO 22:3919-29 Mu- induced allele; CRS2-associated factor; mutant has defect in chloroplast splicing. Barkan lab, unpublished. CAF1 and CAF2 are required for different cp introns.
crp1 7L crp1-1 pale yellow green lacks cyt b6f complex; reduced PSI AF073522 Barkan et al, 1994, EMBO 13: 3170-3181; Fisk et al 1999 EMBO 18: 2621-2630 Missing monocistronic petD and petB RNAs. petA, and petD protein synthesis defective. CRP1 is a PPR protein
crs1 1L crs1-1 pale green specifically lacks the cp ATP synthase; leaky allele AF290414 Jenkins et al, 1997, Plant Cell 9: 283-296; Till et al, 2001, RNA 1227-1238 defect in splicing the group II intron in the cp atpF gene
crs1 crs1-2 very pale yellow green global loss of cp-encoded proteins; null allele AF290414 Jenkins et al, 1997, Plant Cell 9: 283-296; Till et al, 2001, RNA 1227-1238 in addition to splicing defect, has global cp translation defect
crs2 8S between isu1401A and bn19.11 crs2-1 ivory null allele AF225708 Jenkins et al, 1997, Plant Cell 9: 283-296; Jenkins and Barkan, 2001, EMBO 20, 872-879 defect in splicing most group IIB introns in the cp
crs2 crs2-2 crs* very pale yellow green leaky allele AF225708 Jenkins et al, 1997, Plant Cell 9: 283-296; Jenkins and Barkan, 2001, EMBO 20, 872-879 crs* was identified in two families (crs*A and crs*B), which may be identical; but this is not confirmed
csr1 csr1-1 very pale yellow green AJ549215 Yukari Asakuraa et al, The Plant Cell, Vol. 16, 201-214, January 2004 cp FtsY homolog; all three alleles have Mu insertions
csy1 csy1-1 very pale yellow AF039304 Roy and Barkan, 1998, J. Cell Bio 141: 385-395 encodes chloroplast SecY homolog; all three alleles have Mu insertions, predicted nulls
hcf38 5L hcf38-1 pale green low PSII, cyt b6f, PSI, ATP synthase, slightly low Rubisco Barkan et al, 1986, EMBO 5: 1421-1427 cp RNA metabolism defect. Isolated by Don Miles.
pet2 pet2-1 hcf125 pg specifically lacks the cyt b6f complex Voelker and Barkan, 1995, Mol. Gene. Genet. 249: 507-514 PSII reduced when plants grown in sunlight. Synthesis of PetA, PetB, PetC, PetD normal
ppr2 ppr2-4 ivory AY278988 Pascale M. Williams et al, 2003, The Plant Journal Volume 36, Issue 5, Page 675-686 Mu insertion in a gene encoding a cp-localized PPR protein.
tha1 tha1-1 hcf137 pale green low PSI,PSII, cyt b6f U71123 Voelker and Barkan, 1995, Mol. Gene. Genet. 249: 507-514; Voelker et al, 1997, Genetics, 145: 467-478. encodes chloroplast SecA homolog
tha4 1L tha4-1 slightly pale green low PSI, PSII, cyt b6f AF145755 Walker et al, 1999, J. Cell Biol. 147: 267-275. defect in delta pathway for thylakoid targeting
tha5 9s tha5-2 pale green low PSI, PSII, cyt b6f Barkan lab, unpublished defect in cpSec pathway for thylakoid targeting; not cpSecA or cpSecY.
tha8 tha8-1 pale green low PSI, PSII, cyt b6f AY530730 Monde and Barkan, in preparation defect in delta pathway for thylakoid targeting

Seed may be requested from either request@pml.uoregon.edu , or the maize co-op.